Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli PR70(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Q182L | the mutant has significantly lower binding and glycosylase activities for adenine:guanine and adenine:7,8-dihydro-8-oxo-guanine mismatches than the wild type enzyme. The mutant has substantially increased affinity towards thymine:guanine, however, it does not exhibit any thymine/guanine or thymine/7,8-dihydro-8-oxo-guanine glycosylase activity | Escherichia coli |
V45A | the mutant has significantly lower binding and glycosylase activities for adenine:guanine and adenine:7,8-dihydro-8-oxo-guanine mismatches than the wild type enzyme. The mutant has substantially increased affinity towards thymine:guanine, however, it does not exhibit any thymine/guanine or thymine/7,8-dihydro-8-oxo-guanine glycosylase activity | Escherichia coli |
V45A/Q182L | the mutant has similar binding affinities to thymine/guanine as the wild type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phosphocellulose column chromatography, hydroxylapatite column chromatography, heparin column chromatography, and Hitrap-S column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O | - |
Escherichia coli | ? | - |
? | |
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:thymine mismatch + H2O | low activity | Escherichia coli | ? | - |
? | |
DNA containing a guanine:adenine mismatch + H2O | - |
Escherichia coli | ? | - |
? | |
DNA containing a thymine:guanine mismatch + H2O | low activity | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mutY | - |
Escherichia coli |